{"product_id":"promega-n2014","title":"Promega, N2014, NanoBiT® PPI Starter Systems","description":"\u003cp\u003eSensitive Detection of Protein Interactions in Live Cells\u003cbr\u003e\nDetects interactions at low expression levels and allows real-time kinetic analysis in live cells\u003cbr\u003e\nReversible protein complementation reporter to analyze protein association and disassociation\u003cbr\u003e\nImproved sensitivity and accuracy over split firefly luciferase\u003cbr\u003e\nSpecifications：\u003cbr\u003e\npBiT1.1-C [TK\/LgBiT] Vector\tN196A\t1 × 20μg\u003cbr\u003e\npBiT2.1-C [TK\/SmBiT] Vector\tN197A\t1 × 20μg\u003cbr\u003e\npBiT1.1-N [TK\/LgBiT] Vector\tN198A\t1 × 20μg\u003cbr\u003e\npBiT2.1-N [TK\/SmBiT] Vector\tN199A\t1 × 20μg\u003cbr\u003e\nNanoBiT® Negative Control Vector\tN202A\t1 × 20μg\u003cbr\u003e\nLgBiT-PRKAR2A Control Vector\tN203A\t1 × 20μg\u003cbr\u003e\nSmBiT-PRKACA Control Vector\tN204A\t1 × 20μg\u003cbr\u003e\nNano-Glo® Live Cell Substrate\tN205A\t2 × 125μl\u003cbr\u003e\nNano-Glo® LCS Dilution Buffer\tN206A\t2 × 2.5ml\u003cbr\u003e\npFC34K LgBiT TK-neo Flexi® Vector\tN192A\t1 × 20μg\u003cbr\u003e\npFC36K SmBiT TK-neo Flexi® Vector\tN193A\t1 × 20μg\u003cbr\u003e\npFN33K LgBiT TK-neo Flexi® Vector\tN194A\t1 × 20μg\u003cbr\u003e\npFN35K SmBiT TK-neo Flexi® Vector\tN195A\t1 × 20μg\u003cbr\u003e\nNanoBiT® Negative Control Vector\tN202A\t1 × 20μg\u003cbr\u003e\nLgBiT-PRKAR2A Control Vector\tN203A\t1 × 20μg\u003cbr\u003e\nSmBiT-PRKACA Control Vector\tN204A\t1 × 20μg\u003cbr\u003e\nNano-Glo® Live Cell Substrate\tN205A\t2 × 125μl\u003cbr\u003e\nNano-Glo® LCS Dilution Buffer\tN206A\t2 × 2.5ml\u003cbr\u003e\nFRB-LgBiT Control Vector\tN200A\t1 × 20μg\u003cbr\u003e\nFKBP-SmBiT Control Vector\tN201A\t1 × 20μg\u003cbr\u003e\nIn contrast to many split systems, the LgBiT:SmBiT interaction is reversible, allowing the detection of rapidly dissociating proteins. PPI dynamics can be followed in real time in living cells using the Nano-Glo® Live Cell Reagent, a non-lytic detection reagent containing the cell-permeable furimazine substrate. Advantages over split systems include better sensitivity, reversibility, the ability to create fusions to peptides or small, structurally stable protein domains, and the capability for real-time measurement of protein interactions.\u003cbr\u003e\nThe poster\u003cbr\u003e\nMonitoring Intracellular Protein Interactions using NanoBiT Binary Technology\u003cbr\u003e\nprovides more details, including data illustrating these capabilities.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835699810473,"sku":"N2014","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-n2014","provider":"Iright","version":"1.0","type":"link"}