{"product_id":"promega-n5200","title":"Promega, N5200, NanoBRET® 590 Dyes","description":"\u003cp\u003eDevelop Novel NanoBRET® Tracers to Enable Target Engagement Assays for New Targets\u003cbr\u003e\nLeverage the same NanoBRET®-branded energy acceptors used to create most Promega NanoBRET® tracers\u003cbr\u003e\nCouple NanoBRET® 590 SE Dyes to base compounds containing a reactive amine group\u003cbr\u003e\nCreate three unique tracer structures from a common starting material using the three linker variants of the NanoBRET® 590 SE Dye\u003cbr\u003e\nSynthesize tracers with an amine orthogonal coupling chemistry using Azide-Alkyne Click chemistry and the NanoBRET® 590 Azide Dye\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nNanoBRET® 590 SE\tN500A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 SE\tN500B\t1 × 25mg\u003cbr\u003e\nNanoBRET® 590 Azide\tN510A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 PEG(O4)-SE\tN520A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 SE\tN500A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 PEG(O4)-SE\tN520A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 C4 SE\tN530A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 C4 SE\tN530A\t1 × 5mg\u003cbr\u003e\nNanoBRET® 590 C4 SE\tN530B\t1 × 25mg\u003cbr\u003e\nThe NanoBRET® Target Engagement (TE) Assay measures compound binding at select target proteins within intact cells. This target engagement assay is based on the NanoBRET® System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET® Target Engagement Assay analyzes the apparent affinity of test compounds by competitive displacement of a NanoBRET® tracer reversibly bound to a NanoLuc® fusion protein in a cell.\u003cbr\u003e\nWe have a number of TE assays already developed for a large number of protein targets from a variety of protein classes. If there is no existing NanoBRET® TE Assay for your target of interest, use the NanoBRET® 590 Dyes to develop novel tracers to enable NanoBRET® TE assays for new targets.\u003cbr\u003e\nDNA-encoded libraries (DELs) provide unmatched chemical diversity and starting points for novel drug modalities. An article in\u003cbr\u003e\nCell Chemical Biology\u003cbr\u003e\npresents a workflow that exploits the bifunctional attributes of DEL ligands as a platform to generate BRET probes for live-cell target-engagement studies. The combination of DEL and BRET technology accelerates hit-to-lead studies in a live-cell setting.\u003cbr\u003e\nFor more information, see: Teske, K.A.\u003cbr\u003e\net al.\u003cbr\u003e\n(2023) DELs enable the development of BRET probes for target engagement studies in cells.\u003cbr\u003e\nCell Chem. Biol.\u003cbr\u003e\n30\u003cbr\u003e\n, 1–12.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835640959145,"sku":"N5200","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-n5200","provider":"Iright","version":"1.0","type":"link"}