{"product_id":"promega-nd2690","title":"Promega, ND2690, NanoBRET® Ubiquitination Starter Kit","description":"\u003cp\u003eLive-Cell BRET-Based Assay to Measure Protein Ubiquitination\u003cbr\u003e\nGeneral ubiquitination assay to measure changes in the relative level of target protein ubiquitination\u003cbr\u003e\nPerform endpoint or live-cell kinetic analysis to determine protein ubiquitination dynamics\u003cbr\u003e\nAssay ubiquitination on ectopic or endogenously expressed proteins\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980A\t1 × 20μl\u003cbr\u003e\npNLF1-N [CMV\/Hygro] Vector\tN135A\t1 × 20μg\u003cbr\u003e\npNLF1-C [CMV\/Hygro] Vector\tN136A\t1 × 20μg\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157A\t1 × 50μl\u003cbr\u003e\nNanoLuc®-BRD4 FL Fusion Vector\tN169A\t1 × 20μg\u003cbr\u003e\nHaloTag®-Ubiquitin Fusion Vector\tN272A\t1 × 20μg\u003cbr\u003e\nRegulating overall cellular protein homeostasis is critical for maintaining cell health and often altered by cellular treatments or disease states. Most proteins and their abundance are regulated via the ubiquitin proteasome system (UPS), which uses ubiquitin conjugation to signal proteins to be trafficked to the proteasome for degradation. Ubiquitination on any given target can vary in levels, mono- and poly-ubiquitination, and mediated through a variety of amino acid linkages.\u003cbr\u003e\nThe NanoBRET® Ubiquitination Starter Kit provides the tools to create target-specific live-cell ubiquitin assays that globally measure all types of ubiquitination on a target protein. The assays can be used to measure dynamic increases or decreases in the relative levels of target protein ubiquitination following cellular treatments, such as small molecules or pathway inducers, that would influence protein stabilization or degradation, respectively. These assays can be particularly useful for studying targeted degradation compounds because the bioluminescent resonance energy transfer ratio of the NanoBRET™®signal means you can investigate protein ubiquitination while simultaneously monitoring target protein levels to assess degradation.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835696599209,"sku":"ND2690","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-nd2690","provider":"Iright","version":"1.0","type":"link"}