{"product_id":"promega-np4050","title":"Promega, NP4050, NanoBRET® Target Engagement K192 Kinase Selectivity System","description":"\u003cp\u003eProfile Selectivity Across a Diverse Kinase Panel in Live Cells\u003cbr\u003e\nProfile compound cellular selectivity against a panel of 192 human kinases, broadly representing the kinome\u003cbr\u003e\nOptimized, addition-only cellular protocol provides quantitative compound occupancy data for 192 full-length kinases in a single experiment\u003cbr\u003e\nK192 System provides the transfection-ready, arrayed K192 Kinase Vector Panel and a single NanoBRET® TE Kinase Assay\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157D\t1 × 3.3ml\u003cbr\u003e\nTracer Dilution Buffer\tN219B\t1 × 50ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235C\t1 × 1.1ml\u003cbr\u003e\nNanoBRET® Tracer K-10\tN264B\t10 × 300μl\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate A\tNP401A\t2 × 1 each\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate B\tNP402A\t2 × 1 each\u003cbr\u003e\nAdhesive Foil Plate Seals\tNP4000\t1 × 1 each\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157D\t1 × 3.3ml\u003cbr\u003e\nTracer Dilution Buffer\tN219B\t1 × 50ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235C\t1 × 1.1ml\u003cbr\u003e\nNanoBRET® Tracer K-10\tN264B\t10 × 300μl\u003cbr\u003e\nTransfection Carrier DNA\tE488B\t1 × 100μg\u003cbr\u003e\npNL1.1.CMV[\tN109A\t1 × 20μg\u003cbr\u003e\nNanoLuc®-HIPK2 Fusion Vector\tNV322A\t1 × 20μg\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate A\tNP401A\t2 × 1 each\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate B\tNP402A\t2 × 1 each\u003cbr\u003e\nAdhesive Foil Plate Seals\tNP4000\t1 × 1 each\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate A\tNP401A\t2 × 1 each\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate B\tNP402A\t2 × 1 each\u003cbr\u003e\nAdhesive Foil Plate Seals\tNP4000\t1 × 1 each\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate A\tNP401A\t1 × 1 each\u003cbr\u003e\nNanoBRET® TE K192 Vector Plate B\tNP402A\t1 × 1 each\u003cbr\u003e\nAdhesive Foil Plate Seals\tNP4000\t1 × 1 each\u003cbr\u003e\nThe NanoBRET® Target Engagement (TE) K192 Kinase Selectivity System can quantitatively measure intracellular compound occupancy against a panel of 192 kinases in a single experiment. The system relies on the\u003cbr\u003e\nNanoBRET® TE technology\u003cbr\u003e\n, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET® TE Tracer as the energy acceptor. The K192 Kinase Selectivity System is a live-cell method using an addition-only, multiwell plate format with a single NanoBRET® TE Kinase Assay. The NanoBRET® TE K192 Kinase Selectivity System is optimized for each kinase to provide quantitative occupancy data, enabling cellular profiling of compound-kinase interactions.\u003cbr\u003e\nDr. Martin Matzuk explains the importance of NanoBRET® TE in their research.\u003cbr\u003e\nSee how the NanoBRET® TE K192 Kinase Selectivity System has been optimized for a 384-well plate format, allowing for higher throughput and more efficient compound screening per kit.\u003cbr\u003e\nThe Cell Chemical Biology publication is the original demonstration of how NanoBRET® TE could be used for broad kinase selectivity profiling in live cells. This publication illustrates the first live-cell method to quantitatively profile compound occupancy against hundreds of kinases.\u003cbr\u003e\nThe STAR Protocols publication improves upon the Cell Chemical Biology work and is the basis for the NanoBRET® TE K192 Kinase Selectivity System. The authors show how a single NanoBRET® TE Kinase Assay can be used to profile compound engagement across 192 full-length kinases in a single live-cell experiment using a simple workflow.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835640697001,"sku":"NP4050","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-np4050","provider":"Iright","version":"1.0","type":"link"}