{"product_id":"promega-np5100","title":"Promega, NP5100, NanoBRET® Target Engagement CDK Selectivity Systems","description":"\u003cp\u003eProfile Compound Selectivity for Cyclin-Dependent Kinases (CDKs) in Live Cells\u003cbr\u003e\nDetermine compound or inhibitor affinity and occupancy against up to 16 CDKs\u003cbr\u003e\nEach NanoBRET® TE CDK Selectivity System uses a simple, addition-only microplate workflow and provides a CDK Vector Panel (A or B) and a NanoBRET® TE Kinase Assay\u003cbr\u003e\nTwo transfection-ready NanoBRET® TE CDK vector panels (A and B) with 8 individual pairings of CDK-NanoLuc® fusion vector with cyclin\/carrier DNA\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nCDK1\/CCNB1 Vector Mix\tNP501A\t1 × 120μl\u003cbr\u003e\nCDK2\/CCNE1 Vector Mix\tNP502A\t1 × 120μl\u003cbr\u003e\nCDK3\/CCNE1 Vector Mix\tNP503A\t1 × 120μl\u003cbr\u003e\nCDK4\/CCND3 Vector Mix\tNP504A\t1 × 120μl\u003cbr\u003e\nCDK5\/CDK5R1 Vector Mix\tNP505A\t1 × 120μl\u003cbr\u003e\nCDK6\/CCDN1 Vector Mix\tNP506A\t1 × 120μl\u003cbr\u003e\nCDK7\/Carrier Vector Mix\tNP507A\t1 × 120μl\u003cbr\u003e\nCDK9\/CCNK Vector Mix\tNP508A\t1 × 120μl\u003cbr\u003e\nTransfection Carrier DNA\tE488B\t1 × 100μg\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157C\t1 × 330μl\u003cbr\u003e\nTracer Dilution Buffer\tN219A\t1 × 5ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235B\t1 × 110μl\u003cbr\u003e\nNanoBRET® Tracer K-10\tN264B\t1 × 300μl\u003cbr\u003e\nLIMK1-NanoLuc® Fusion Vector\tNV339A\t1 × 20μg\u003cbr\u003e\nCDK1\/CCNB1 Vector Mix\tNP501A\t1 × 120μl\u003cbr\u003e\nCDK2\/CCNE1 Vector Mix\tNP502A\t1 × 120μl\u003cbr\u003e\nCDK3\/CCNE1 Vector Mix\tNP503A\t1 × 120μl\u003cbr\u003e\nCDK4\/CCND3 Vector Mix\tNP504A\t1 × 120μl\u003cbr\u003e\nCDK5\/CDK5R1 Vector Mix\tNP505A\t1 × 120μl\u003cbr\u003e\nCDK6\/CCDN1 Vector Mix\tNP506A\t1 × 120μl\u003cbr\u003e\nCDK7\/Carrier Vector Mix\tNP507A\t1 × 120μl\u003cbr\u003e\nCDK9\/CCNK Vector Mix\tNP508A\t1 × 120μl\u003cbr\u003e\nCDK10\/CCNL2 Vector Mix\tNP511A\t1 × 120μl\u003cbr\u003e\nCDK14\/CCNY Vector Mix\tNP512A\t1 × 120μl\u003cbr\u003e\nCDK15\/CCNY Vector Mix\tNP513A\t1 × 120μl\u003cbr\u003e\nCDK16\/CCNY Vector Mix\tNP514A\t1 × 120μl\u003cbr\u003e\nCDK17\/CCNY Vector Mix\tNP515A\t1 × 120μl\u003cbr\u003e\nCDK18\/CCNY Vector Mix\tNP516A\t1 × 120μl\u003cbr\u003e\nCDKL2\/Carrier Vector Mix\tNP517A\t1 × 120μl\u003cbr\u003e\nCDK20\/CCNH Vector Mix\tNP518A\t1 × 120μl\u003cbr\u003e\nTransfection Carrier DNA\tE488B\t1 × 100μg\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157C\t1 × 330μl\u003cbr\u003e\nTracer Dilution Buffer\tN219A\t1 × 5ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235B\t1 × 110μl\u003cbr\u003e\nNanoBRET® Tracer K-10\tN264B\t1 × 300μl\u003cbr\u003e\nLIMK1-NanoLuc® Fusion Vector\tNV339A\t1 × 20μg\u003cbr\u003e\nCDK10\/CCNL2 Vector Mix\tNP511A\t1 × 120μl\u003cbr\u003e\nCDK14\/CCNY Vector Mix\tNP512A\t1 × 120μl\u003cbr\u003e\nCDK15\/CCNY Vector Mix\tNP513A\t1 × 120μl\u003cbr\u003e\nCDK16\/CCNY Vector Mix\tNP514A\t1 × 120μl\u003cbr\u003e\nCDK17\/CCNY Vector Mix\tNP515A\t1 × 120μl\u003cbr\u003e\nCDK18\/CCNY Vector Mix\tNP516A\t1 × 120μl\u003cbr\u003e\nCDKL2\/Carrier Vector Mix\tNP517A\t1 × 120μl\u003cbr\u003e\nCDK20\/CCNH Vector Mix\tNP518A\t1 × 120μl\u003cbr\u003e\nThe NanoBRET® Target Engagement (TE) CDK Selectivity Systems quantitatively measure intracellular compound affinity and occupancy against two panels of 8 CDKs each, allowing you to measure up to 16 CDKs in a single experiment. Each vector panel contains 8 individual tubes of CDK-NanoLuc® fusion vectors that are premixed with either a cyclin expression vector or carrier DNA for optimal transfections.\u003cbr\u003e\nThe CDK Selectivity Systems rely on the\u003cbr\u003e\nNanoBRET® TE technology\u003cbr\u003e\n, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET® TE Tracer as the energy acceptor. The CDK Selectivity Systems use an addition-only, multiwell plate format with a single NanoBRET® TE Kinase Assay.\u003cbr\u003e\nIn this study, the NanoBRET® TE method was used to profile a collection of 46 clinically advanced CDK inhibitors and tool molecules in live cells. The results confirmed the binding of advanced clinical compounds and revealed new drug-CDK interactions. These new findings offer the potential to repurpose drugs for lesser-studied CDK family members. This paper highlights the importance of determining drug selectivity across the CDK family in live cells. The NanoBRET® TE CDK Selectivity Systems expanded on this work, simplifying the workflow to use a single NanoBRET® TE Assay (K-10) and ready-to-use CDK Vector Panels.\u003cbr\u003e\nWells, C.I.\u003cbr\u003e\net al.\u003cbr\u003e\n(2020) Quantifying CDK inhibitor selectivity in live cells.\u003cbr\u003e\nNat. Comm.\u003cbr\u003e\n11\u003cbr\u003e\n, 2743.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835640664233,"sku":"NP5100","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-np5100","provider":"Iright","version":"1.0","type":"link"}