{"product_id":"promega-w1333","title":"Promega, W1333, Lumit® Immunoassay Cellular Systems","description":"\u003cp\u003eNo-Wash, One-Plate Immunoassay for Cellular Analytes\u003cbr\u003e\nNew Lumit® Lysis Buffer II included\u003cbr\u003e\n! Offers enhanced performance across a wide range of cellular targets\u003cbr\u003e\nNew Application Notes\u003cbr\u003e\navailable for\u003cbr\u003e\nmore validated targets\u003cbr\u003e\nSet 1: Lumit® Anti-Mouse Ab-LgBiT\/Lumit® Anti-Rabbit Ab-SmBiT\u003cbr\u003e\nSet 2: Lumit® Anti-Mouse Ab-SmBiT\/Lumit® Anti-Rabbit Ab-LgBiT\u003cbr\u003e\nStarter Kit: Includes all four Set 1 and Set 2 Lumit® Secondary Antibodies; primary antibodies supplied by the user\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nPhospho-4E-BP1 (Ser 65)\tmTor\tSet 2 (W1331)\u003cbr\u003e\nAKT\tPI3K\/mTOR\/AKT\tSet 1 (W1201)\u003cbr\u003e\nPhospho-AKT (Ser 473)\tPI3K\/mTOR\/AKT\tSet 1 (W1201)\u003cbr\u003e\nBCL6\tB-cell signaling\tSet 1 (W1201)\u003cbr\u003e\nβ-catenin (Human)\tWNT\tSet 2 (W1331)\u003cbr\u003e\nPhospho-β-catenin (Thr 41\/Ser 45)\tWNT\tSet 2 (W1331)\u003cbr\u003e\nBurton's Tyrosine Kinase (BTK)\tB-cell Receptor (BCR)\tSet 1 (W1201)\u003cbr\u003e\nPhospho-BTK (Tyr 223)\tB-cell Receptor (BCR)\tSet 1 (W1201)\u003cbr\u003e\nPhospho-CHK1 (Ser 317)\tDDR Pathway\tSet 1 (W1201)\u003cbr\u003e\nPhospho-c-Jun (Ser 63)\tJNK\tSet 1 (W1201)\u003cbr\u003e\nPhospho-c-MET (Tyr 1234\/1235)\tc-MET\tSet 1 (W1201)\u003cbr\u003e\nPhospho-c-MET (Tyr 1349)\tc-MET\tSet 1 (W1201)\u003cbr\u003e\nCREB\tPKA\/CREB\tSet 1 (W1201)\u003cbr\u003e\nPhospho-CREB (Ser 133)\tPKA\/CREB\tSet 1 (W1201)\u003cbr\u003e\nEGFR\tEGFR\tSet 1 (W1201)\u003cbr\u003e\nPhospho-EGFR (Tyr 1068)\tEGFR\tSet 1 (W1201)\u003cbr\u003e\nPhospho-EGFR (Tyr 1173)\tEGFR\tSet 1 (W1201)\u003cbr\u003e\nER (Estrogen Receptor)\tEstrogen\tSet 1 (W1201)\u003cbr\u003e\nPhospho-ERK1 (Thr 202)\tERK\tSet 1 (W1201)\u003cbr\u003e\nPhospho-GSK1-3β (Ser 9)\tGSK-3β\tSet 1 (W1201)\u003cbr\u003e\nPhospho-H2AX\tDDR Pathway\tSet 1 (W1201)\u003cbr\u003e\nPhospho-HER2 (Tyr 1196)\tHER2\tSet 1 (W1201)\u003cbr\u003e\nPhospho-HER2 (Tyr 1221\/1222)\tHER2\tSet 1 (W1201)\u003cbr\u003e\nIκBα\tNF-κB\tSet 1 (W1201)\u003cbr\u003e\nPhospho-IκBα (Ser 32)\tNF-κB\tSet 1 (W1201)\u003cbr\u003e\nPhospho-JNK (Thr 183\/Tyr 185)\tJNK\tSet 1 (W1201)\u003cbr\u003e\np65\tNF-κB\tSet 2 (W1331)\u003cbr\u003e\nPhospho-p65 (Ser 536)\tNF-κB\tSet 2 (W1331)\u003cbr\u003e\nRb (Retinoblastoma Tumor Suppressor)\tCell Cycle\tSet 2 (W1331)\u003cbr\u003e\nPhospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 780)\tCell Cycle\tSet 2 (W1331)\u003cbr\u003e\nPhospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 807\/811)\tCell Cycle\tSet 2 (W1331)\u003cbr\u003e\nRibosomal Protein S6\tPI3K\/mTOR\/AKT\tSet 1 (W1201)\u003cbr\u003e\nPhospho-Ribosomal Protein S6 (Ser 240\/244)\tPI3K\/mTOR\/AKT\tSet 1 (W1201)\u003cbr\u003e\nSmad1\tBMP\tSet 1 (W1201)\u003cbr\u003e\nPhospho-Smad1 (Ser 463\/465)\tBMP\tSet 1 (W1201)\u003cbr\u003e\nSmad2\tTGF-β\tSet 2 (W1331)\u003cbr\u003e\nPhospho-Smad2 (Ser 465\/467)\tTGF-β\tSet 2 (W1331)\u003cbr\u003e\nSTAT1\tJAK\/STAT\tSet 1 (W1201)\u003cbr\u003e\nPhospho-STAT1 (Tyr 701)\tJAK\/STAT\tSet 1 (W1201)\u003cbr\u003e\nPhospho-STAT1 (Ser 727)\tJAK\/STAT\tSet 1 (W1201)\u003cbr\u003e\nPhospho-STAT2 (Tyr 690)\tJAK\/STAT\tSet 1 (W1201)\u003cbr\u003e\nSTAT 3\tJAK\/STAT\tSet 1 (W1201)\u003cbr\u003e\nPhospho-STAT 3 (Tyr 705)\tJAK\/STAT\tSet 1 (W1201)\u003cbr\u003e\nAKT\tPROTAC MS-21\tSet 1 (W1201)\u003cbr\u003e\nB-cell lymphoma 6 protein (BCL-6)\tBI-3802\tSet 1 (W1201)\u003cbr\u003e\nBRD4\tPROTACs ARV-771,  dBET6\tSet 1 (W1201)\u003cbr\u003e\nBurton's Tyrosine Kinase (BTK)\tPROTAC MT-802\tSet 1 (W1201)\u003cbr\u003e\nER (Estrogen Receptor)\tFulvestrant\tSet 1 (W1201)\u003cbr\u003e\nSMARCA2 (BRM)\tPROTAC ACBI-1\tSee Application Note for details\u003cbr\u003e\nSMARCA4 (BRG1)\tPROTAC ACBI-1\tSet 2 (W1331)\u003cbr\u003e\nSTAT3\tPROTAC SD-36\tSet 1 (W1201)\u003cbr\u003e\nDigitonin\tG944C\t1 × 50μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103A\t1 × 200μl\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115A\t1 × 1.5ml\u003cbr\u003e\nLumit® Anti-Mouse Ab-LgBiT\tW102A\t1 × 30μl\u003cbr\u003e\nLumit® Anti-Rabbit Ab-SmBiT\tW103A\t1 × 30μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t1 × 1ml\u003cbr\u003e\nGF-AFC Substrate\tG608A-C\t1 × 10μl\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310A\t1 × 500μl\u003cbr\u003e\nDigitonin\tG944D\t1 × 500μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103B\t2 × 1ml\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115B\t1 × 15ml\u003cbr\u003e\nLumit® Anti-Mouse Ab-LgBiT\tW102B\t1 × 300μl\u003cbr\u003e\nLumit® Anti-Rabbit Ab-SmBiT\tW103B\t1 × 300μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t1 × 1ml\u003cbr\u003e\nGF-AFC Substrate\tG608A-C\t2 × 10μl\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310B\t1 × 5ml\u003cbr\u003e\nDigitonin\tG944D\t10 × 500μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103B\t20 × 1ml\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115B\t10 × 15ml\u003cbr\u003e\nLumit® Anti-Mouse Ab-LgBiT\tW102B\t10 × 300μl\u003cbr\u003e\nLumit® Anti-Rabbit Ab-SmBiT\tW103B\t10 × 300μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t10 × 1ml\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310B\t10 × 5ml\u003cbr\u003e\nDigitonin\tG944C\t1 × 50μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103A\t1 × 200μl\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115A\t1 × 1.5ml\u003cbr\u003e\nLumit® Anti-Rabbit Ab-LgBiT\tW104A\t1 × 30μl\u003cbr\u003e\nLumit® Anti-Mouse Ab-SmBiT\tW105A\t1 × 30μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t1 × 1ml\u003cbr\u003e\nGF-AFC Substrate\tG608A-C\t1 × 10μl\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310A\t1 × 500μl\u003cbr\u003e\nDigitonin\tG944D\t1 × 500μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103B\t2 × 1ml\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115B\t1 × 15ml\u003cbr\u003e\nLumit® Anti-Rabbit Ab-LgBiT\tW104B\t1 × 300μl\u003cbr\u003e\nLumit® Anti-Mouse Ab-SmBiT\tW105B\t1 × 300μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t1 × 1ml\u003cbr\u003e\nGF-AFC Substrate\tG608A-C\t2 × 10μl\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310B\t1 × 5ml\u003cbr\u003e\nDigitonin\tG944D\t10 × 500μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103B\t20 × 1ml\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115B\t10 × 15ml\u003cbr\u003e\nLumit® Anti-Rabbit Ab-LgBiT\tW104B\t10 × 300μl\u003cbr\u003e\nLumit® Anti-Mouse Ab-SmBiT\tW105B\t10 × 300μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t10 × 1ml\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310B\t10 × 5ml\u003cbr\u003e\nDigitonin\tG944C\t2 × 50μl\u003cbr\u003e\nLumit® Detection Substrate C\tVB103A\t2 × 200μl\u003cbr\u003e\nLumit® Immunoassay Buffer C, 10X\tVB115A\t2 × 1.5ml\u003cbr\u003e\nLumit® Anti-Mouse Ab-LgBiT\tW102A\t1 × 30μl\u003cbr\u003e\nLumit® Anti-Rabbit Ab-SmBiT\tW103A\t1 × 30μl\u003cbr\u003e\nLumit® Anti-Rabbit Ab-LgBiT\tW104A\t1 × 30μl\u003cbr\u003e\nLumit® Anti-Mouse Ab-SmBiT\tW105A\t1 × 30μl\u003cbr\u003e\nAntibody Dilution Buffer\tW108A\t1 × 1ml\u003cbr\u003e\nGF-AFC Substrate\tG608A-C\t1 × 10μl\u003cbr\u003e\nLumit® Lysis Buffer II, 10X\tVB310A\t2 × 500μl\u003cbr\u003e\nThe Lumit® Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect a wide range of proteins, phosphoproteins or small molecules of interest, provided the appropriate primary antibodies (supplied by the user) are available. We recently developed a new lysis buffer (Lumit® Lysis Buffer II), which broadens the range of cellular targets (see table below) that can be interrogated using the Lumit® Immunoassay Cellular System.\u003cbr\u003e\nAlso available are\u003cbr\u003e\nComplete Assays\u003cbr\u003e\n, which include sets of antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK as well as several others. These are available as early access materials.\u003cbr\u003e\nTo save you time, we have evaluated a number of common targets using commercially available primary antibodies. We have expanded the number of Application Notes to include targets that are now accessible due to the new lysis buffer. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.\u003cbr\u003e\nNote:\u003cbr\u003e\nThe total number of assays per kit will vary based on final well volume.\u003cbr\u003e\nFor standard well volumes:\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835642532009,"sku":"W1333","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-w1333","provider":"Iright","version":"1.0","type":"link"}