{"product_id":"proteintech-10104-1-ap","title":"Proteintech, 10104-1-AP, GPAA1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GPAA1 (10104-1-AP) by Proteintech is a Polyclonal antibody targeting GPAA1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n10104-1-AP targets GPAA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HuH-7 cells, HepG2 cells,  mouse brain tissue,  rat brain tissue,  MCF-7 cells,  MKN-45 cells\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0152 Product name: Recombinant human GPAA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 62-321 aa of BC004129 Sequence: EQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRG Predict reactive species\nFull Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)\nCalculated Molecular Weight: 68 kDa\nObserved Molecular Weight: 60-68 kDa\nGenBank Accession Number: BC004129\nGene Symbol: GPAA1\nGene ID (NCBI): 8733\nRRID: AB_2263708\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43292\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869409136809,"sku":"10104-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10104-1-ap","provider":"Iright","version":"1.0","type":"link"}