{"product_id":"proteintech-10166-1-ap","title":"Proteintech, 10166-1-AP, DOPA decarboxylase\/DDC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DOPA decarboxylase\/DDC (10166-1-AP) by Proteintech is a Polyclonal antibody targeting DOPA decarboxylase\/DDC in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10166-1-AP targets DOPA decarboxylase\/DDC in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SH-SY5Y cells, mouse kidney tissue,  mouse brain tissue,  rat kidney tissue,  PC-12 cells\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: mouse kidney tissue, human liver cancer tissue,  rat kidney tissue,  rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily (PMID: 38467937). DDC is an enzyme that converts levodopa into dopamine, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 28751068). DDC plays an important role in the dopaminergic system and participates in the uptake and decarboxylation of amine precursors in the peripheral tissues (PMID: 28100850). It is reported that DDC may be a novel and potential marker for the detection of peritoneal micrometastases of gastric cancer (PMID: 14760382).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0219 Product name: Recombinant human DDC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 121-389 aa of BC008366 Sequence: WLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFE Predict reactive species\nFull Name: dopa decarboxylase (aromatic L-amino acid decarboxylase)\nCalculated Molecular Weight: 54 kDa\nObserved Molecular Weight: 48-50 kDa\nGenBank Accession Number: BC008366\nGene Symbol: DOPA decarboxylase\nGene ID (NCBI): 1644\nRRID: AB_2088937\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P20711\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868924170409,"sku":"10166-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10166-1-ap","provider":"Iright","version":"1.0","type":"link"}