{"product_id":"proteintech-10246-2-ap","title":"Proteintech, 10246-2-AP, CPLX1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CPLX1 (10246-2-AP) by Proteintech is a Polyclonal antibody targeting CPLX1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n10246-2-AP targets CPLX1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, rat brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nComplexin 1 (CPLX1) is a member of the complexin\/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, macaque\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0387 Product name: Recombinant human CPLX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 3-134 aa of BC002471 Sequence: FVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTVIKYLPGPLQDMLKK Predict reactive species\nFull Name: complexin 1\nCalculated Molecular Weight: 15 kDa\nObserved Molecular Weight: 15-20 kDa\nGenBank Accession Number: BC002471\nGene Symbol: CPLX1\nGene ID (NCBI): 10815\nRRID: AB_2084033\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O14810\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868932886697,"sku":"10246-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10246-2-ap","provider":"Iright","version":"1.0","type":"link"}