{"product_id":"proteintech-10247-2-ap","title":"Proteintech, 10247-2-AP, GNB1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GNB1 (10247-2-AP) by Proteintech is a Polyclonal antibody targeting GNB1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n10247-2-AP targets GNB1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Jurkat cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: human pancreas cancer tissue,  human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGNB1(Guanine nucleotide-binding protein G(I)\/G(S)\/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0313 Product name: Recombinant human GNB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-339 aa of BC004186 Sequence: SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW Predict reactive species\nFull Name: guanine nucleotide binding protein (G protein), beta polypeptide 1\nCalculated Molecular Weight: 37 kDa\nObserved Molecular Weight: 37 kDa\nGenBank Accession Number: BC004186\nGene Symbol: GNB1\nGene ID (NCBI): 2782\nRRID: AB_2111820\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P62873\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868941373609,"sku":"10247-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10247-2-ap","provider":"Iright","version":"1.0","type":"link"}