{"product_id":"proteintech-10262-1-ap","title":"Proteintech, 10262-1-AP, PPP4C Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PPP4C (10262-1-AP) by Proteintech is a Polyclonal antibody targeting PPP4C in WB, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10262-1-AP targets PPP4C in WB, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  Jurkat cells,  mouse kidney tissue\nPositive IP detected in: mouse kidney tissue\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine\/threonine protein phosphatases (PP) are intimately involved in this process. Serine\/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel\/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel\/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel\/NF-kappa B, which is in contrast to other protein phosphatases.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0356 Product name: Recombinant human PPP4C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC001416 Sequence: MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL Predict reactive species\nFull Name: protein phosphatase 4 (formerly X), catalytic subunit\nCalculated Molecular Weight: 35 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC001416\nGene Symbol: PPP4C\nGene ID (NCBI): 5531\nRRID: AB_2300020\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P60510\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868959559849,"sku":"10262-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10262-1-ap","provider":"Iright","version":"1.0","type":"link"}