{"product_id":"proteintech-10298-1-ap","title":"Proteintech, 10298-1-AP, Lamin A\/C Polyclonal antibody","description":"Size: 150ul\nThe Lamin A\/C (10298-1-AP) by Proteintech is a Polyclonal antibody targeting Lamin A\/C in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n10298-1-AP targets Lamin A\/C in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, HEK-293 cells,  HUVEC cells,  mouse ovary tissue,  SKOV-3 cells,  HeLa cells,  A375 cells,  C6 cells,  NIH\/3T3 cells\nPositive IP detected in: HeLa cells, A375 cells\nPositive IHC detected in: mouse heart tissue, human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, HeLa cells\nPositive FC (Intra) detected in: HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLamin A\/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A\/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A\/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A\/C.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, monkey, duck\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0408 Product name: Recombinant human Lamin A\/C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 26-198 aa of BC003162 Sequence: ITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQ Predict reactive species\nFull Name: lamin A\/C\nCalculated Molecular Weight: 65 kDa\nObserved Molecular Weight: 65 kDa, 70 kDa\nGenBank Accession Number: BC003162\nGene Symbol: Lamin A\/C\nGene ID (NCBI): 4000\nENSEMBL Gene ID: ENSG00000160789\nRRID: AB_2296961\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P02545\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868821967017,"sku":"10298-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10298-1-ap","provider":"Iright","version":"1.0","type":"link"}