{"product_id":"proteintech-10737-1-ap","title":"Proteintech, 10737-1-AP, TPM3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TPM3 (10737-1-AP) by Proteintech is a Polyclonal antibody targeting TPM3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10737-1-AP targets TPM3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, HEK-293 cells,  HeLa cells,  human heart tissue,  rat heart tissue,  mouse skeletal muscle tissue,  rat skeletal muscle tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1171 Product name: Recombinant human TPM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC008407 Sequence: MMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISEELDHALNDMTSI Predict reactive species\nFull Name: tropomyosin 3\nCalculated Molecular Weight: 33 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC008407\nGene Symbol: TPM3\nGene ID (NCBI): 7170\nRRID: AB_2205671\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P06753\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868908998825,"sku":"10737-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10737-1-ap","provider":"Iright","version":"1.0","type":"link"}