{"product_id":"proteintech-10744-1-ap","title":"Proteintech, 10744-1-AP, MRE11 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MRE11 (10744-1-AP) by Proteintech is a Polyclonal antibody targeting MRE11 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10744-1-AP targets MRE11 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HT-1376 cells,  HeLa cells,  HepG2 cells\nPositive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMre11 (meiotic recombination 11), also named as Mre11A, is a component of the MRN( MRE11\/RAD50\/NBS1) complex which is a versatile complex, playing a key role in the sensing, processing, and repair of DSBs. MRE11 possesses both endonuclease and 3'-5' exonuclease activity. It contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with CtIP (CtBP [C terminal binding protein] interacting protein) to initiate end resection. It's reported that MRE11 is over-expressed in breast cancers. (PMID:38128537; 22914783)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1105 Product name: Recombinant human MRE11A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC005241 Sequence: MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKED Predict reactive species\nFull Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)\nCalculated Molecular Weight: 81 kDa\nObserved Molecular Weight: 81 kDa\nGenBank Accession Number: BC005241\nGene Symbol: MRE11A\nGene ID (NCBI): 4361\nRRID: AB_2145118\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P49959\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868898218153,"sku":"10744-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10744-1-ap","provider":"Iright","version":"1.0","type":"link"}