{"product_id":"proteintech-10854-1-ap","title":"Proteintech, 10854-1-AP, JP45\/JSRP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe JP45\/JSRP1 (10854-1-AP) by Proteintech is a Polyclonal antibody targeting JP45\/JSRP1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n10854-1-AP targets JP45\/JSRP1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human skeletal muscle tissue\nPositive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nJSRP1 (Junctional sarcoplasmic reticulum protein 1), also known as JP-45, is an integral protein of the junctional face membrane of the skeletal muscle sarcoplasmic reticulum (SR), and interacts with calsequestrin and the skeletal-muscle dihydropyridine receptor Cav1. JP-45 is an important protein for functional expression of voltage-dependent calcium channels. Involvement of JP-45 in the molecular mechanism underlying skeletal muscle excitation-contraction coupling has been reported. Although the predicated molecular weight of JP-45 is 36 kDa, it appears a 45-kDa band in western blotting in most cases.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1300 Product name: Recombinant human JP45; JSRP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC021201 Sequence: MSMTTRAWEELDGGLGSCQALEDHSALAETQEDRASATPRLADSGSVPHDSQVAEGPSVDTRPKKMEKEPAARGTPGTGKERLKAGASPRSVPARKKAQTAPPLQPPPPPPALSEELPWGDLSLNKCLVLASLVALLGSAFQLCRDAVPGEAALQARVPEPWVPPSSAPREPSSPLPKFEAQAPPSAPPAPRAEAEVRPKIPGSREAAENDEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEERPRAAREPREALPQRWESREGGHRPWARDSRDAEPRKKQAWVSPRRPDEEQRPGSRQKLRAGKGRD Predict reactive species\nFull Name: junctional sarcoplasmic reticulum protein 1\nCalculated Molecular Weight: 36 kDa, 45 kDa\nObserved Molecular Weight: 30 kDa\nGenBank Accession Number: BC021201\nGene Symbol: JP45\/JSRP1\nGene ID (NCBI): 126306\nRRID: AB_2129459\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96MG2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887689257129,"sku":"10854-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-10854-1-ap","provider":"Iright","version":"1.0","type":"link"}