{"product_id":"proteintech-11041-1-ap","title":"Proteintech, 11041-1-AP, B4GALT3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe B4GALT3 (11041-1-AP) by Proteintech is a Polyclonal antibody targeting B4GALT3 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n11041-1-AP targets B4GALT3 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells,  NIH\/3T3 cells\nPositive IHC detected in: human liver cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells, A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:20-1:300\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nB4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1487 Product name: Recombinant human B4GALT3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC009985 Sequence: MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNS Predict reactive species\nFull Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3\nCalculated Molecular Weight: 44 kDa\nObserved Molecular Weight: 44 kDa\nGenBank Accession Number: BC009985\nGene Symbol: B4GALT3\nGene ID (NCBI): 8703\nRRID: AB_2290072\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O60512\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869394063529,"sku":"11041-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-11041-1-ap","provider":"Iright","version":"1.0","type":"link"}