{"product_id":"proteintech-11091-1-ap","title":"Proteintech, 11091-1-AP, EMAP II Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EMAP II (11091-1-AP) by Proteintech is a Polyclonal antibody targeting EMAP II in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n11091-1-AP targets EMAP II in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, A549 cells,  SGC-7901 cells,  SH-SY5Y cells,  U-251 cells,  mouse brain tissue\nPositive IP detected in: SGC-7901 cells\nPositive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1560 Product name: Recombinant human SCYE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC014051 Sequence: MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK Predict reactive species\nFull Name: small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)\nCalculated Molecular Weight: 34 kDa\nObserved Molecular Weight: 34 kDa and 68 kDa\nGenBank Accession Number: BC014051\nGene Symbol: EMAP II\nGene ID (NCBI): 9255\nRRID: AB_2182590\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q12904\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868965589161,"sku":"11091-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-11091-1-ap","provider":"Iright","version":"1.0","type":"link"}