{"product_id":"proteintech-11118-1-ap","title":"Proteintech, 11118-1-AP, MEST Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MEST (11118-1-AP) by Proteintech is a Polyclonal antibody targeting MEST in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n11118-1-AP targets MEST in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human testis tissue,  mouse testis tissue,  PC-3 cells\nPositive IP detected in: PC-3 cells\nPositive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt\/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1617 Product name: Recombinant human MEST protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC011908 Sequence: MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWD Predict reactive species\nFull Name: mesoderm specific transcript homolog (mouse)\nCalculated Molecular Weight: 38 kDa\nObserved Molecular Weight: 34 kDa\nGenBank Accession Number: BC011908\nGene Symbol: MEST\nGene ID (NCBI): 4232\nRRID: AB_2143740\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q5EB52\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868907098281,"sku":"11118-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-11118-1-ap","provider":"Iright","version":"1.0","type":"link"}