{"product_id":"proteintech-11281-1-ap","title":"Proteintech, 11281-1-AP, EIF4G3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF4G3 (11281-1-AP) by Proteintech is a Polyclonal antibody targeting EIF4G3 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n11281-1-AP targets EIF4G3 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, HEK-293 cells,  A549 cells,  HeLa cells,  HepG2 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:10-1:100\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein  migrated at approximately 70  kDa and\/or  250 kDa in SDS-PAGE.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1811 Product name: Recombinant human EIF4G3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 197-515 aa of BC030578 Sequence: SQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC Predict reactive species\nFull Name: eukaryotic translation initiation factor 4 gamma, 3\nCalculated Molecular Weight: 177 kDa\nObserved Molecular Weight: 70 kDa, 250 kDa\nGenBank Accession Number: BC030578\nGene Symbol: EIF4G3\nGene ID (NCBI): 8672\nRRID: AB_2877754\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43432\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868979974313,"sku":"11281-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-11281-1-ap","provider":"Iright","version":"1.0","type":"link"}