{"product_id":"proteintech-11703-1-ap","title":"Proteintech, 11703-1-AP, MCM5 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MCM5 (11703-1-AP) by Proteintech is a Polyclonal antibody targeting MCM5 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n11703-1-AP targets MCM5 in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A375 cells, HeLa cells,  Jurkat cells,  mouse thymus tissue,  rat thymus tissue\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: mouse thymus tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMinichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2271 Product name: Recombinant human MCM5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 388-734 aa of BC000142 Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK Predict reactive species\nFull Name: minichromosome maintenance complex component 5\nCalculated Molecular Weight: 82 kDa\nObserved Molecular Weight: 82-88 kDa\nGenBank Accession Number: BC000142\nGene Symbol: MCM5\nGene ID (NCBI): 4174\nRRID: AB_2235162\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P33992\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868867940521,"sku":"11703-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-11703-1-ap","provider":"Iright","version":"1.0","type":"link"}