{"product_id":"proteintech-11859-1-ap","title":"Proteintech, 11859-1-AP, BRD3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BRD3 (11859-1-AP) by Proteintech is a Polyclonal antibody targeting BRD3 in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications with reactivity to human samples\n11859-1-AP targets BRD3 in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HeLa cells,  COLO 320 cells,  HepG2 cells,  Jurkat cells,  K-562 cells,  MCF-7 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human prostate cancer tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse testis tissue\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). \nThe antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2433 Product name: Recombinant human BRD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC032124 Sequence: MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAM Predict reactive species\nFull Name: bromodomain containing 3\nCalculated Molecular Weight: 556 aa, 61 kDa\nObserved Molecular Weight: 95-105 kDa\nGenBank Accession Number: BC032124\nGene Symbol: BRD3\nGene ID (NCBI): 8019\nRRID: AB_2065902\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15059\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868859977897,"sku":"11859-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-11859-1-ap","provider":"Iright","version":"1.0","type":"link"}