{"product_id":"proteintech-12121-1-ap","title":"Proteintech, 12121-1-AP, RAD23B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RAD23B (12121-1-AP) by Proteintech is a Polyclonal antibody targeting RAD23B in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12121-1-AP targets RAD23B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, HEK-293 cells,  HeLa cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and\/or in altering chromatin structure to allow access by damage-processing enzymes.  RAD23B displays aberrant mobility in SDS-polyacrylamide gels, as it is typically detected as a 58- to 60-kDa protein despite a predicted size of 42 kDa (PMID: 8692695, 12052895).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2763 Product name: Recombinant human RAD23B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-409 aa of BC020973 Sequence: MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSSTTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATSPTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNPDRAVEYLLMGIPGDRESQAVVDPPQAASTGVPQSSAVAAAAATTTATTTTTSSGGHPLEFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED Predict reactive species\nFull Name: RAD23 homolog B (S. cerevisiae)\nCalculated Molecular Weight: 409 aa, 43 kDa\nObserved Molecular Weight: 58 kDa\nGenBank Accession Number: BC020973\nGene Symbol: RAD23B\nGene ID (NCBI): 5887\nRRID: AB_10641048\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P54727\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868959920297,"sku":"12121-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-12121-1-ap","provider":"Iright","version":"1.0","type":"link"}