{"product_id":"proteintech-12345-1-ap","title":"Proteintech, 12345-1-AP, DYNC1H1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DYNC1H1 (12345-1-AP) by Proteintech is a Polyclonal antibody targeting DYNC1H1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n12345-1-AP targets DYNC1H1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Jurkat cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: mouse brain tissue, human breast cancer tissue,  human normal colon,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2999 Product name: Recombinant human KIAA0325 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 590-989 aa of BC021297 Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE Predict reactive species\nFull Name: dynein, cytoplasmic 1, heavy chain 1\nCalculated Molecular Weight: 4646 aa, 532 kDa\nObserved Molecular Weight: 532 kDa\nGenBank Accession Number: BC021297\nGene Symbol: DYNC1H1\nGene ID (NCBI): 1778\nRRID: AB_2261765\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14204\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868837499049,"sku":"12345-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-12345-1-ap","provider":"Iright","version":"1.0","type":"link"}