{"product_id":"proteintech-12395-1-ap","title":"Proteintech, 12395-1-AP, MAT1A\/MAT2A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MAT1A\/MAT2A (12395-1-AP) by Proteintech is a Polyclonal antibody targeting MAT1A\/MAT2A in WB, IHC, ELISA applications with reactivity to human, mouse, rat, arabidopsis samples\n12395-1-AP targets MAT1A\/MAT2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, arabidopsis samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse liver tissue, arabidopsis whole plant tissue,  HCT 116 cells,  HeLa cells,  HepG2 cells,  rat liver tissue\nPositive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMethionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and can recognize both of MAT1A and MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, arabidopsis\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3076 Product name: Recombinant human MAT1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-395 aa of BC018359 Sequence: MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF Predict reactive species\nFull Name: methionine adenosyltransferase I, alpha\nCalculated Molecular Weight: 395 aa, 44 kDa\nObserved Molecular Weight: 47 kDa, 49 kDa\nGenBank Accession Number: BC018359\nGene Symbol: MAT1A\nGene ID (NCBI): 4143\nRRID: AB_2266186\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q00266\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868942618793,"sku":"12395-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-12395-1-ap","provider":"Iright","version":"1.0","type":"link"}