{"product_id":"proteintech-12411-1-ap","title":"Proteintech, 12411-1-AP, PI4KA Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PI4KA (12411-1-AP) by Proteintech is a Polyclonal antibody targeting PI4KA in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n12411-1-AP targets PI4KA in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse brain tissue,  human brain tissue,  MCF-7 cells,  NIH-3T3 cells (RNAi),  rat brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPI4KA, also named as PIK4, PIK4CA, belongs to the PI3\/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3062 Product name: Recombinant human PI4KA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1745-2044 aa of BC018120 Sequence: YLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY Predict reactive species\nFull Name: phosphatidylinositol 4-kinase, catalytic, alpha\nCalculated Molecular Weight: 2044 aa, 231 kDa\nObserved Molecular Weight: 231 kDa\nGenBank Accession Number: BC018120\nGene Symbol: PI4KA\nGene ID (NCBI): 5297\nRRID: AB_2268237\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P42356\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868913717417,"sku":"12411-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-12411-1-ap","provider":"Iright","version":"1.0","type":"link"}