{"product_id":"proteintech-12781-1-ap","title":"Proteintech, 12781-1-AP, CBLB Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CBLB (12781-1-AP) by Proteintech is a Polyclonal antibody targeting CBLB in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n12781-1-AP targets CBLB in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  A549 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: mouse colon tissue, human kidney tissue,  human lung tissue,  human placenta tissue,  human spleen tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCBLB(E3 ubiquitin-protein ligase CBL-B) is also named RNF56 and plays a necessary role in preventing the inappropriate activation of NF-B in response to lipopolysaccharide and polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and various types of cell stimulation. It also has a crucial role in preventing chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing 109, 90, and 86 kDa, and observed molecular weight at 110-130 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3491 Product name: Recombinant human CBLB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC032851 Sequence: MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDH Predict reactive species\nFull Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b\nCalculated Molecular Weight: 982 aa, 109 kDa\nObserved Molecular Weight: 110-130 kDa\nGenBank Accession Number: BC032851\nGene Symbol: CBLB\nGene ID (NCBI): 868\nRRID: AB_2275326\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13191\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868893008041,"sku":"12781-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-12781-1-ap","provider":"Iright","version":"1.0","type":"link"}