{"product_id":"proteintech-13624-1-ap","title":"Proteintech, 13624-1-AP, CAMKK1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CAMKK1 (13624-1-AP) by Proteintech is a Polyclonal antibody targeting CAMKK1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCalcium\/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)\/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda  and  involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1\/PKB that inhibits pro-apoptotic BAD\/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory.This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 .\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4534 Product name: Recombinant human CAMKK1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-348 aa of BC031647 Sequence: MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSST Predict reactive species\nFull Name: calcium\/calmodulin-dependent protein kinase kinase 1, alpha\nCalculated Molecular Weight: 520 aa, 57 kDa\nObserved Molecular Weight: 63 kDa\nGenBank Accession Number: BC031647\nGene Symbol: CAMKK1\nGene ID (NCBI): 84254\nRRID: AB_2068403\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8N5S9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46885783929001,"sku":"13624-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-13624-1-ap","provider":"Iright","version":"1.0","type":"link"}