{"product_id":"proteintech-13635-1-ap","title":"Proteintech, 13635-1-AP, POLI Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe POLI (13635-1-AP) by Proteintech is a Polyclonal antibody targeting POLI in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n13635-1-AP targets POLI in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse testis tissue, human heart tissue,  rat testis tissue\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates \"G\" opposite template \"T\" more frequently than incorporates the correct \"A.\" [PMID:21304950] It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. [PMID:17609217]. It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress[PMID:15199127].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, mosquito\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4563 Product name: Recombinant human POLI protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC032662 Sequence: MELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLASLLNRVCQDGRKPH Predict reactive species\nFull Name: polymerase (DNA directed) iota\nCalculated Molecular Weight: 715 aa, 80 kDa\nObserved Molecular Weight: 87 kDa\nGenBank Accession Number: BC032662\nGene Symbol: POLI\nGene ID (NCBI): 11201\nRRID: AB_2167009\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UNA4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868926857385,"sku":"13635-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-13635-1-ap","provider":"Iright","version":"1.0","type":"link"}