{"product_id":"proteintech-13762-1-ap","title":"Proteintech, 13762-1-AP, AMBRA1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AMBRA1 (13762-1-AP) by Proteintech is a Polyclonal antibody targeting AMBRA1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n13762-1-AP targets AMBRA1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, HepG2 cells,  HT-1080 cells,  human brain tissue,  mouse brain tissue,  U2OS cells\nPositive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application. The full length of Ambra1 is about 140 kDa, with the C-terminal fragment measuring approximately 100 kDa and the N-terminal fragment measuring approximately 50 kDa (PMID: 22441670).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4735 Product name: Recombinant human AMBRA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 906-1208 aa of BC045609 Sequence: NVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTETLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTVHCELTNNNHLLDGGSSRGDAAGPRGEPRNR Predict reactive species\nFull Name: autophagy\/beclin-1 regulator 1\nCalculated Molecular Weight: 1208 aa, 133 kDa\nObserved Molecular Weight: 110kDa, 130-135 kDa\nGenBank Accession Number: BC045609\nGene Symbol: AMBRA1\nGene ID (NCBI): 55626\nRRID: AB_10642007\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9C0C7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868873281705,"sku":"13762-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-13762-1-ap","provider":"Iright","version":"1.0","type":"link"}