{"product_id":"proteintech-13979-1-ap","title":"Proteintech, 13979-1-AP, TLK2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TLK2 (13979-1-AP) by Proteintech is a Polyclonal antibody targeting TLK2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n13979-1-AP targets TLK2 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, HeLa cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTousled-like kinases (TLKs) are a family of serine\/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5022 Product name: Recombinant human TLK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 512-831 aa of BC044925 Sequence: DHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN Predict reactive species\nFull Name: tousled-like kinase 2\nCalculated Molecular Weight: 772 aa, 88 kDa\nObserved Molecular Weight: 85 kDa\nGenBank Accession Number: BC044925\nGene Symbol: TLK2\nGene ID (NCBI): 11011\nRRID: AB_2203887\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86UE8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869437317289,"sku":"13979-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-13979-1-ap","provider":"Iright","version":"1.0","type":"link"}