{"product_id":"proteintech-14412-1-ap","title":"Proteintech, 14412-1-AP, PEG10 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PEG10 (14412-1-AP) by Proteintech is a Polyclonal antibody targeting PEG10 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n14412-1-AP targets PEG10 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, L02 cells,  MCF-7 cells,  MDA-MB-231 cells,  T-47D cells\nPositive IHC detected in: mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPaternally expressed imprinted gene 10 (PEG10) is an evolutionarily conserved HERV gene belonging to the Ty3\/Gypsy family of retrotransposons. The mRNA of PEG10 encodes at least two protein isoforms: the major PEG10 protein product reading frame 1 (RF1-PEG10, ~50 kDa) and reading frames 1 and 2 (RF1\/RF2-PEG10, ~100 kDa), which are translated by a typical retroviral-1 ribosomal frameshift mechanism. PEG10 is strongly expressed in placenta, ovary and testis as well as somatic adult organs including brain, kidney and lung . Researches on mice suggest that PEG10 plays an important role in placenta formation and adipocyte differentiation, and its knockout can cause embryonic lethality.(PMID: 30123090, PMID: 32244497)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5754 Product name: Recombinant human PEG10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC050659 Sequence: MTERRRDELSEEINNLREKVMKQSEENNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPEKFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSSPAGNSPAPL Predict reactive species\nFull Name: paternally expressed 10\nCalculated Molecular Weight: 80 kDa\nObserved Molecular Weight: 50 kDa, 100 kDa\nGenBank Accession Number: BC050659\nGene Symbol: PEG10\nGene ID (NCBI): 23089\nRRID: AB_10694427\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86TG7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868926529705,"sku":"14412-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-14412-1-ap","provider":"Iright","version":"1.0","type":"link"}