{"product_id":"proteintech-14583-1-ap","title":"Proteintech, 14583-1-AP, RPL7 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RPL7 (14583-1-AP) by Proteintech is a Polyclonal antibody targeting RPL7 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n14583-1-AP targets RPL7 in WB, IHC, IF\/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  mouse kidney tissue\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6104 Product name: Recombinant human RPL7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-248 aa of BC006095 Sequence: MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN Predict reactive species\nFull Name: ribosomal protein L7\nCalculated Molecular Weight: 29 kDa\nObserved Molecular Weight: 29-30 kDa\nGenBank Accession Number: BC006095\nGene Symbol: RPL7\nGene ID (NCBI): 6129\nRRID: AB_2254049\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P18124\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868914340009,"sku":"14583-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-14583-1-ap","provider":"Iright","version":"1.0","type":"link"}