{"product_id":"proteintech-14685-1-ap","title":"Proteintech, 14685-1-AP, PAK4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PAK4 (14685-1-AP) by Proteintech is a Polyclonal antibody targeting PAK4 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n14685-1-AP targets PAK4 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, HeLa cells,  PC-3 cells,  rat skin tissue\nPositive IP detected in: mouse liver tissue\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42\/p21 (PMID:11278822).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5981 Product name: Recombinant human PAK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC025282 Sequence: MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVK Predict reactive species\nFull Name: p21 protein (Cdc42\/Rac)-activated kinase 4\nCalculated Molecular Weight: 64 kDa\nObserved Molecular Weight: 64 kDa\nGenBank Accession Number: BC025282\nGene Symbol: PAK4\nGene ID (NCBI): 10298\nRRID: AB_2158610\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O96013\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868872003753,"sku":"14685-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-14685-1-ap","provider":"Iright","version":"1.0","type":"link"}