{"product_id":"proteintech-15097-1-ap","title":"Proteintech, 15097-1-AP, ATP6V1B2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ATP6V1B2 (15097-1-AP) by Proteintech is a Polyclonal antibody targeting ATP6V1B2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n15097-1-AP targets ATP6V1B2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, A549 cells,  HeLa cells,  mouse brain tissue,  mouse heart tissue,  mouse skeletal muscle tissue,  K-562 cells,  L02 cells,  rat brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha\/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, pig, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7174 Product name: Recombinant human ATP6V1B2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 162-511 aa of BC003100 Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH Predict reactive species\nFull Name: ATPase, H+ transporting, lysosomal 56\/58kDa, V1 subunit B2\nCalculated Molecular Weight: 57 kDa\nObserved Molecular Weight: 56-58 kDa\nGenBank Accession Number: BC003100\nGene Symbol: ATP6V1B2\nGene ID (NCBI): 526\nRRID: AB_2243297\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P21281\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868895989929,"sku":"15097-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15097-1-ap","provider":"Iright","version":"1.0","type":"link"}