{"product_id":"proteintech-15157-1-ap","title":"Proteintech, 15157-1-AP, FUCA2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FUCA2 (15157-1-AP) by Proteintech is a Polyclonal antibody targeting FUCA2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n15157-1-AP targets FUCA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human heart tissue, mouse stomach tissue,  rat stomach tissue\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: human liver tissue,  human hepatocirrhosis tissue,  human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAlpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7250 Product name: Recombinant human FUCA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 124-467 aa of BC003060 Sequence: SGAKYIVLTSKHHEGFTLWGSEYSWNWNAIDEGPKRDIVKELEVAIRNRTDLRFGLYYSLFEWFHPLFLEDESSSFHKRQFPVSKTLPELYELVNNYQPEVLWSDGDGGAPDQYWNSTGFLAWLYNESPVRGTVVTNDRWGAGSICKHGGFYTCSDRYNPGHLLPHKWENCMTIDKLSWGYRREAGISDYLTIEELVKQLVETVSCGGNLLMNIGPTLDGTISVVFEERLRQVGSWLKVNGEAIYETYTWRSQNDTVTPDVWYTSKPKEKLVYAIFLKWPTSGQLFLGHPKAILGATEVKLLGHGQPLNWISLEQNGIMVELPQLTIHQMPCKWGWALALTNVI Predict reactive species\nFull Name: fucosidase, alpha-L- 2, plasma\nCalculated Molecular Weight: 54 kDa\nObserved Molecular Weight: 54-60 kDa\nGenBank Accession Number: BC003060\nGene Symbol: FUCA2\nGene ID (NCBI): 2519\nRRID: AB_2104956\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BTY2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869685239977,"sku":"15157-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15157-1-ap","provider":"Iright","version":"1.0","type":"link"}