{"product_id":"proteintech-15180-1-ap","title":"Proteintech, 15180-1-AP, CA12 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CA12 (15180-1-AP) by Proteintech is a Polyclonal antibody targeting CA12 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n15180-1-AP targets CA12 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, MCF-7 cells\nPositive IHC detected in: human brown disease, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide.  Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7107 Product name: Recombinant human CA12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-293 aa of BC000278 Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI Predict reactive species\nFull Name: carbonic anhydrase XII\nCalculated Molecular Weight: 39 kDa\nObserved Molecular Weight: 44 kDa, 75-80 kDa\nGenBank Accession Number: BC000278\nGene Symbol: CA12\nGene ID (NCBI): 771\nRRID: AB_2065826\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43570\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868873347241,"sku":"15180-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15180-1-ap","provider":"Iright","version":"1.0","type":"link"}