{"product_id":"proteintech-15331-1-ap","title":"Proteintech, 15331-1-AP, MVD Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MVD (15331-1-AP) by Proteintech is a Polyclonal antibody targeting MVD in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n15331-1-AP targets MVD in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HCT 116 cells, rat liver tissue,  HepG2 cells,  K-562 cells\nPositive IHC detected in: human colon tissue, human lung cancer tissue,  human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A549 cells, A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7255 Product name: Recombinant human MVD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC000011 Sequence: MASEKPLAAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGREEDVGQPRLQACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAGYACLAYTLARVYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAISWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPPGSNGDTFLKGLQVRPAPLSAELQAALAMEPTPGGVKYIIVTQVGPGPQILDDPCAHLLGPDGLPKPAA Predict reactive species\nFull Name: mevalonate (diphospho) decarboxylase\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 66-74 kDa, 45 kDa, 37 kDa\nGenBank Accession Number: BC000011\nGene Symbol: MVD\nGene ID (NCBI): 4597\nRRID: AB_2147439\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P53602\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868984791209,"sku":"15331-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15331-1-ap","provider":"Iright","version":"1.0","type":"link"}