{"product_id":"proteintech-15621-1-ap","title":"Proteintech, 15621-1-AP, RNF40 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RNF40 (15621-1-AP) by Proteintech is a Polyclonal antibody targeting RNF40 in WB, ELISA applications with reactivity to human, mouse samples\n15621-1-AP targets RNF40 in WB, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, mouse liver tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8047 Product name: Recombinant human RNF40 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 700-1001 aa of BC006133 Sequence: VDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS Predict reactive species\nFull Name: ring finger protein 40\nCalculated Molecular Weight: 114 kDa\nObserved Molecular Weight: 130-140 kDa\nGenBank Accession Number: BC006133\nGene Symbol: RNF40\nGene ID (NCBI): 9810\nRRID: AB_2253949\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O75150\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887787069609,"sku":"15621-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15621-1-ap","provider":"Iright","version":"1.0","type":"link"}