{"product_id":"proteintech-15678-1-ap","title":"Proteintech, 15678-1-AP, CDC45L Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CDC45L (15678-1-AP) by Proteintech is a Polyclonal antibody targeting CDC45L in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n15678-1-AP targets CDC45L in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells, HEK-293 cells,  HeLa cells,  Jurkat cells,  mouse thymus tissue\nPositive IP detected in: K-562 cells\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, chicken\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8150 Product name: Recombinant human CDC45L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 217-566 aa of BC010022 Sequence: LWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS Predict reactive species\nFull Name: CDC45 cell division cycle 45-like (S. cerevisiae)\nCalculated Molecular Weight: 64 kDa\nObserved Molecular Weight: 60-66 kDa\nGenBank Accession Number: BC010022\nGene Symbol: CDC45L\nGene ID (NCBI): 8318\nRRID: AB_10642704\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O75419\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868939473065,"sku":"15678-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15678-1-ap","provider":"Iright","version":"1.0","type":"link"}