{"product_id":"proteintech-15723-1-ap","title":"Proteintech, 15723-1-AP, P3H2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe P3H2 (15723-1-AP) by Proteintech is a Polyclonal antibody targeting P3H2 in WB, IP, IHC, ELISA applications with reactivity to human,  mouse samples\n15723-1-AP targets P3H2 in WB, IP, IHC, ELISA applications and shows reactivity with human,  mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: L02 cells, HEK-293 cells,  mouse placenta tissue,  mouse kidney tissue,  human placenta tissue\nPositive IP detected in: mouse kidney tissue\nPositive IHC detected in: human liver tissue, human kidney tissue,  human placenta tissue,  human testis tissue,  human skin tissue,  human brain tissue,  human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nP3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8444 Product name: Recombinant human LEPREL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 179-527 aa of BC005029 Sequence: ASIEAREDLTMFVKRHKLESELIKSAAEGLGFSYTEPNYWIRYGGRQDENRVPSGVNVEGAEVHGFSMGKKLSPKIDRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSEEQCRELHSVASGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKSGYEGRVPLKSARLFYDISEKARRIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFEGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTKGKRCAVALWFTLDPLYRELERIQADEVIAILDQEQQGKHELNINPKDEL Predict reactive species\nFull Name: leprecan-like 1\nCalculated Molecular Weight: 708aa,81 kDa; 527aa,60 kDa\nObserved Molecular Weight: 80 kDa\nGenBank Accession Number: BC005029\nGene Symbol: LEPREL1\nGene ID (NCBI): 55214\nRRID: AB_2136486\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8IVL5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869479620777,"sku":"15723-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15723-1-ap","provider":"Iright","version":"1.0","type":"link"}