{"product_id":"proteintech-15882-1-ap","title":"Proteintech, 15882-1-AP, PPP2R1A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PPP2R1A (15882-1-AP) by Proteintech is a Polyclonal antibody targeting PPP2R1A in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n15882-1-AP targets PPP2R1A in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, A431 cells,  mouse kidney tissue,  rat liver tissue,  SMMC-7721 cells,  mouse liver tissue,  L02 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: mouse kidney tissue, human kidney tissue,  human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPPP2R1A belongs to the phosphatase 2A regulatory subunit A family. PPP2R1A encodes the α-isoform of the scaffolding subunit of the serine\/threonine protein phosphatase 2A (PP2A) holoenzyme. This tumour suppressor complex is involved in growth and survival pathways and PPP2R1A mutations in endometrial high-grade serous carcinomas are a frequent and potentially targetable feature of this disease(PMID: 21381030). The PPP2R1A gene has 86% identity with the PPP2R1B gene(PMID:7681822).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8661 Product name: Recombinant human PPP2R1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 239-589 aa of BC001537 Sequence: DLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA Predict reactive species\nFull Name: protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform\nCalculated Molecular Weight: 589 aa, 65 kDa\nObserved Molecular Weight: 65 kDa\nGenBank Accession Number: BC001537\nGene Symbol: PPP2R1A\nGene ID (NCBI): 5518\nRRID: AB_2237574\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P30153\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868913881257,"sku":"15882-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-15882-1-ap","provider":"Iright","version":"1.0","type":"link"}