{"product_id":"proteintech-16195-1-ap","title":"Proteintech, 16195-1-AP, Claudin 4-specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Claudin 4-specific (16195-1-AP) by Proteintech is a Polyclonal antibody targeting Claudin 4-specific in WB, IHC, IF-P, IP, ELISA applications with reactivity to human samples\n16195-1-AP targets Claudin 4-specific in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-29 cells, MCF-7 cells,  MDA-MB-231 cells,  human placenta tissue\nPositive IP detected in: human placenta tissue\nPositive IHC detected in: human breast cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human breast cancer tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nClaudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa)  proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against Claudin 4. Claudin 4 is overexpressed in a variety of cancers. It can be used as a predictor of recurrent malignant pleural effusion in patients with advanced non-small cell lung cancer (PMID: 38629624).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, pig, canine, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: claudin 4\nCalculated Molecular Weight: 22 kDa\nObserved Molecular Weight: 22 kDa\nGenBank Accession Number: NM_001305\nGene Symbol: Claudin 4\nGene ID (NCBI): 1364\nRRID: AB_2082969\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O14493\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868839235753,"sku":"16195-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-16195-1-ap","provider":"Iright","version":"1.0","type":"link"}