{"product_id":"proteintech-16552-1-ap","title":"Proteintech, 16552-1-AP, SMPDL3B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SMPDL3B (16552-1-AP) by Proteintech is a Polyclonal antibody targeting SMPDL3B in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n16552-1-AP targets SMPDL3B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, HEK-293 cells\nPositive IHC detected in: human kidney tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSMPDL3B (sphingomyelin phosphodiesterase acid-like 3B) is lipid-modulating phosphodiesterase, which is active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9535 Product name: Recombinant human SMPDL3B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-336 aa of BC014444 Sequence: MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWALINSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPKNQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADPGQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHTDSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKV Predict reactive species\nFull Name: sphingomyelin phosphodiesterase, acid-like 3B\nCalculated Molecular Weight: 373aa,42 kDa; 455aa,51 kDa\nObserved Molecular Weight: 42 kDa, 50-65 kDa\nGenBank Accession Number: BC014444\nGene Symbol: SMPDL3B\nGene ID (NCBI): 27293\nRRID: AB_2878276\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q92485\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868973846697,"sku":"16552-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-16552-1-ap","provider":"Iright","version":"1.0","type":"link"}