{"product_id":"proteintech-16848-1-ap","title":"Proteintech, 16848-1-AP, Cytokeratin 1-specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cytokeratin 1-specific (16848-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 1-specific in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples\n16848-1-AP targets Cytokeratin 1-specific in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat skin tissue, mouse skin tissue\nPositive IP detected in: mouse skin tissue\nPositive IHC detected in: rat skin tissue, human cervical cancer tissue,  human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse skin tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKeratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is  a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: keratin 1\nCalculated Molecular Weight: 66 kDa\nObserved Molecular Weight: 67 kDa\nGenBank Accession Number: NM_006121\nGene Symbol: KRT1\nGene ID (NCBI): 3848\nRRID: AB_2134417\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P04264\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868869152937,"sku":"16848-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-16848-1-ap","provider":"Iright","version":"1.0","type":"link"}