{"product_id":"proteintech-17023-1-ap","title":"Proteintech, 17023-1-AP, COL6A1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe COL6A1 (17023-1-AP) by Proteintech is a Polyclonal antibody targeting COL6A1 in WB, IHC, IF, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples\n17023-1-AP targets COL6A1 in WB, IHC, IF, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, HEK-293T cells,  human heart tissue,  human skeletal muscle tissue,  rat heart tissue\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: human colon tissue, human liver tissue,  mouse liver tissue,  human heart tissue,  human hepatocirrhosis tissue,  human kidney tissue,  human placenta tissue,  human testis tissue,  human spleen tissue,  human lung tissue,  human ovary tissue,  human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human colon tissue, mouse skeletal muscle tissue\nPositive IF detected in: mouse skeletal muscle tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:300-1:1200\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF): IF : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nType VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, zebrafish, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10288 Product name: Recombinant human COL6A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 770-1028 aa of BC052575 Sequence: NVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG Predict reactive species\nFull Name: collagen, type VI, alpha 1\nCalculated Molecular Weight: 1028 aa, 109 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC052575\nGene Symbol: COL6A1\nGene ID (NCBI): 1291\nRRID: AB_2229737\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P12109\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868851359913,"sku":"17023-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-17023-1-ap","provider":"Iright","version":"1.0","type":"link"}