{"product_id":"proteintech-17354-1-ap","title":"Proteintech, 17354-1-AP, MKNK2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MKNK2 (17354-1-AP) by Proteintech is a Polyclonal antibody targeting MKNK2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n17354-1-AP targets MKNK2 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse spleen tissue, MCF-7 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMKNK2(MAP kinase-interacting serine\/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser\/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium\/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10546 Product name: Recombinant human MKNK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-158 aa of BC018345 Sequence: MAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLLPPHPCRIHVRPGGLVRTVTVNE Predict reactive species\nFull Name: MAP kinase interacting serine\/threonine kinase 2\nCalculated Molecular Weight: 465 aa, 52 kDa\nObserved Molecular Weight: 47-52 kDa\nGenBank Accession Number: BC018345\nGene Symbol: MKNK2\nGene ID (NCBI): 2872\nRRID: AB_2878395\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9HBH9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868984135849,"sku":"17354-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-17354-1-ap","provider":"Iright","version":"1.0","type":"link"}