{"product_id":"proteintech-17687-1-ap","title":"Proteintech, 17687-1-AP, PJA1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PJA1 (17687-1-AP) by Proteintech is a Polyclonal antibody targeting PJA1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples\n17687-1-AP targets PJA1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, mouse brain tissue,  rat brain tissue,  SH-SY5Y cells\nPositive IP detected in: mouse brain tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag11820 Product name: Recombinant human PJA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 294-643 aa of BC075803 Sequence: ANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL Predict reactive species\nFull Name: praja ring finger 1\nCalculated Molecular Weight: 643 aa, 71 kDa\nObserved Molecular Weight: 71-80 kDa\nGenBank Accession Number: BC075803\nGene Symbol: PJA1\nGene ID (NCBI): 64219\nRRID: AB_2252483\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8NG27\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868935934121,"sku":"17687-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-17687-1-ap","provider":"Iright","version":"1.0","type":"link"}