{"product_id":"proteintech-17720-1-ap","title":"Proteintech, 17720-1-AP, CSK Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CSK (17720-1-AP) by Proteintech is a Polyclonal antibody targeting CSK in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, rat samples\n17720-1-AP targets CSK in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Raji cells, K-562 cells,  HL-60 cells,  Jurkat cells,  rat lung tissue\nPositive IP detected in: HL-60 cells\nPositive IHC detected in: human lung tissue, human skin tissue,  human placenta tissue,  human kidney tissue,  human brain tissue,  human ovary tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12103 Product name: Recombinant human CSK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 83-450 aa of BC106073 Sequence: FHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL Predict reactive species\nFull Name: c-src tyrosine kinase\nCalculated Molecular Weight: 450 aa, 50 kDa\nObserved Molecular Weight: 50 kDa\nGenBank Accession Number: BC106073\nGene Symbol: CSK\nGene ID (NCBI): 1445\nRRID: AB_2084150\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P41240\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868964802729,"sku":"17720-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-17720-1-ap","provider":"Iright","version":"1.0","type":"link"}