{"product_id":"proteintech-18543-1-ap","title":"Proteintech, 18543-1-AP, PDLIM4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PDLIM4 (18543-1-AP) by Proteintech is a Polyclonal antibody targeting PDLIM4 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n18543-1-AP targets PDLIM4 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, K-562 cells\nPositive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:300-1:1200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPDLIM4, also known as RIL, is a member of the ALP\/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:\n12908099).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13424 Product name: Recombinant human PDLIM4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC016765 Sequence: MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRGPSGTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSRDCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERLYCESHAKARVKPPEGYDVVAVYPNAKVELV Predict reactive species\nFull Name: PDZ and LIM domain 4\nCalculated Molecular Weight: 35 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC016765\nGene Symbol: PDLIM4\nGene ID (NCBI): 8572\nRRID: AB_3085565\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P50479\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887755743401,"sku":"18543-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-18543-1-ap","provider":"Iright","version":"1.0","type":"link"}