{"product_id":"proteintech-18900-1-ap","title":"Proteintech, 18900-1-AP, ZO-2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ZO-2 (18900-1-AP) by Proteintech is a Polyclonal antibody targeting ZO-2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n18900-1-AP targets ZO-2 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HEK-293 cells,  HeLa cells,  MCF-7 cells,  mouse heart tissue,  mouse brain tissue,  rat brain tissue,  NIH\/3T3 cells,  C6 cells\nPositive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nZona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.What is the molecular weight of ZO-2? Are there any isoforms of ZO-2? Is ZO-2 post-translationally modified?The molecular weight of ZO-2 is 160 kDa. There are two known isoforms of ZO-2: full-length ZO-2A and ZO-2C lacking the first 23 amino acids (PMID: 10360833). ZO-2 can be phosphorylated and O-glycosylated, which has an impact on its localization (PMID: 23804652).What is the subcellular localization of ZO-2?ZO-2 is present at the cytoplasmic face at cell-cell contact sites: tight junctions, adherens junctions, and gap junctions (PMID: 20224657). ZO-2 contains nuclear localization and nuclear export signals and can actively shuttle between the cytoplasm and nucleus (PMID: 11855865). In the nucleus it can affect the activity of various transcription factors. ZO-2 nuclear localization is most prominent in sparse 2D cell cultures and also in cells subjected to mechanical or chemical stress.What is the tissue expression pattern of ZO-2?ZO-2 is expressed in various tissues in epithelial and endothelial cells. ZO-2 isoforms have tissue specificity, with ZO-2A being abundant in the heart and brain, while ZO-2C is found in the kidney, pancreas, heart, and placenta (PMID: 11018256).What proteins can be used as markers of tight junctions?Zona occludens proteins are often used as tight junction markers. Apart from the antibody against ZO-2, one can use antibodies against ZO-1 (https:\/\/www.ptglab.com\/products\/ZO1-Antibody-21773-1-AP.htm), occludin (https:\/\/www.ptglab.com\/products\/OCLN-Antibody-13409-1-AP.htm), and claudins (e.g., claudin 1:https:\/\/www.ptglab.com\/products\/CLDN1-Antibody-13050-1-AP.htm).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13534 Product name: Recombinant human TJP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-289 aa of BC027592 Sequence: MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREH Predict reactive species\nFull Name: tight junction protein 2 (zona occludens 2)\nCalculated Molecular Weight: 134 kDa\nObserved Molecular Weight: 150 kDa\nGenBank Accession Number: BC027592\nGene Symbol: TJP2\nGene ID (NCBI): 9414\nRRID: AB_2203584\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UDY2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868856176809,"sku":"18900-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/proteintech-18900-1-ap","provider":"Iright","version":"1.0","type":"link"}